Kinetics of copper(II) uptake by apoazurin in complexing media.

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Kinetics of copper(II) uptake by apoazurin in complexing media.

We have studied the kinetics of copper uptake by apoazurin in imidazole and 1-methylimidazole buffers in the pH range of 7-9 with mu = 0.5 M and copper(II) in large excess. The reaction has been monitored by measuring the visible absorbance and circular dichroism as a function of time. The uptake occurs in a stepwise fashion, and at least two intermediates are implicated. Overall, the rate of u...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1983

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)44581-9